Archive for the ‘Practical Course’ Category

Staph pigments competition: Three winners already!

Monday 2 April, 2007

We already have three winners for the Staph pigments competition.

The winners are
1) Samar Galal Ahmed Kabeel (سمر جلال أحمد قابيل) emailed the right answer at 4:28 PM, Cairo Time
2) Mohammed Shehta Mostafa (محمد شحتة عبد النبي مصطفى) posted the right answer at 9:02 PM, Cairo Time
3) Soha Khairy ElMekkawy (سهى خيري المكاوي) handed out a full answer at 3:30 PM, Cairo Time, in the laboratory.

Congratulations.
All others, please keep sending your answers.

Want to learn more about PCR and electrophoresis?

Sunday 25 March, 2007

I have no doubt that many of you did not understand the principle of PCR in the lecture; it is really hard to get it in 20 minutes. I hope that the printed material helped you a little bit better.
Moreover, many of those who understand the principle still cannot imagine how things are performed in practice.
It is very hard to get enough PCR thermocyclers to let you all perform the reaction, but you may enjoy the following links that present illustrations and animations on the principle and methodology of PCR and gel electrophoresis.

A PCR thermocycler looks like this one below:

A typical electrophoresis apparatus is shown here:

Now, more resources:

Electrophoresis:
Principle of electrophoresis (includes a simple animated cartoon)
Principles of gel electrophoresis

PCR:
PCR (The University of Alabama in Huntsville Laboratory for Structural Biology)
Principle of the PCR
PCR animation (The page takes a while to launch but it’s really worth waiting. BEST ANIMATION TO DELIVER THE AMPLIFICATION CONCEPT)
Another good animation

Please suggest more links and I will put them one by one for you…

Diagnostic Virology Prelab: Lecture Notes (March 19)

Monday 19 March, 2007

It’s never too late.
Here are the Diagnostic Virology lecture notes, released few hours before the first lab section of this week.

The notes are available for all. You need no password. I put the lecture notes in two sizes:
1) A “regular” size: 4 pages (PDF file)
2) A “smaller” size: 2 pages (PDF file- same everything, but the font is smaller and the lines are less spaced)

Because PCR is a very important technique and because some of you want to understand it better, I put some more information about PCR in an appendix (PDF file). Obviously, information in this appendix is not for memorization and will not be a part in the exam (to calm down exam-oriented students) but only for those who want to read and learn.

IMPORTANT:
– Please, give me feedback on these lecture notes. Are they clear? long? short? too brief? too detailed? Also please let me know if you find any spelling (or scientific) mistake!

Protected: Enterobacteriaceae (I) Prelab Lecture Notes [To view, use the password provided in the lecture]

Wednesday 14 March, 2007

This content is password protected. To view it please enter your password below:

On the types of hemolysis

Friday 2 March, 2007

One image I found interesting about differentiating the three types of hemolysis:

I have no idea to whom the picture credit should go; I found it in this page from Dr. Victor Nizet’s laboratory’s web site.

One more image:

Check out this link about hemolysis- it is really one of the best.

I will keep reminding you that this site is yours. Please feel free to suggest–by commenting below–any other good pictures you find interesting.


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