Want to learn more about PCR and electrophoresis?

I have no doubt that many of you did not understand the principle of PCR in the lecture; it is really hard to get it in 20 minutes. I hope that the printed material helped you a little bit better.
Moreover, many of those who understand the principle still cannot imagine how things are performed in practice.
It is very hard to get enough PCR thermocyclers to let you all perform the reaction, but you may enjoy the following links that present illustrations and animations on the principle and methodology of PCR and gel electrophoresis.

A PCR thermocycler looks like this one below:

A typical electrophoresis apparatus is shown here:

Now, more resources:

Electrophoresis:
Principle of electrophoresis (includes a simple animated cartoon)
Principles of gel electrophoresis

PCR:
PCR (The University of Alabama in Huntsville Laboratory for Structural Biology)
Principle of the PCR
PCR animation (The page takes a while to launch but it’s really worth waiting. BEST ANIMATION TO DELIVER THE AMPLIFICATION CONCEPT)
Another good animation

Please suggest more links and I will put them one by one for you…

Advertisements

6 Responses to “Want to learn more about PCR and electrophoresis?”

  1. basma mohamed shehata Says:

    Thank you but please I want to say that the sites of lectures of Dr Abdelgawad and Dr hamadallah werenot opened

  2. RKA Says:

    Dear Basma,
    thank you for your inupt.
    I have tested Dr. Abdel Gawad’s lecture page and the page is opening fine (click here).
    If I understood correctly, you could not open the page. Once the page is opened, you can download the files and uncompress them to see the lectures.

    On the other hand, I will transfer your concerns to Dr. Hamdallah Zedan and I hope that any technical issue will be resolved.

  3. s.positive Says:

    thanx alot Dr RKA
    i suggest that u put a small expalanation of PCR by yourself.
    it wil help us more,the animations and the sites r good ,but explanation will does help.
    thank u again.

  4. s.positive Says:

    dear Dr RKA
    is the PCR a method for just amplification of DNA ?
    what about the plasmid cloning method used for production of hormones like insulin?
    can the PCR method be used for the same purpose?

  5. s.positive Says:

    I searched about the relation between PCR& Plasmid cloning and found out that:PCR is often used to amplify the gene, which can then be inserted into a vector (a vector is a piece of DNA which ‘carries’ the gene into the GMO) such as a plasmid (a circular DNA molecule) . The DNA can then be transferred into an organism (the GMO) where the gene and its product can be studied more closely. Expressing a cloned gene (when a gene is expressed the gene product (usually protein or RNA) is produced by the GMO) can also be a way of mass-producing useful proteins, for example medicines or the enzymes.
    GMO= genetically modified organism

  6. s.positive Says:

    dear Dr Ramy

    please make a comment on my last post about plasmid cloning and PCR.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s


%d bloggers like this: