I have no doubt that many of you did not understand the principle of PCR in the lecture; it is really hard to get it in 20 minutes. I hope that the printed material helped you a little bit better.
Moreover, many of those who understand the principle still cannot imagine how things are performed in practice.
It is very hard to get enough PCR thermocyclers to let you all perform the reaction, but you may enjoy the following links that present illustrations and animations on the principle and methodology of PCR and gel electrophoresis.
A PCR thermocycler looks like this one below:
A typical electrophoresis apparatus is shown here:
Now, more resources:
Electrophoresis:
- Principle of electrophoresis (includes a simple animated cartoon)
- Principles of gel electrophoresis
PCR:
- PCR (The University of Alabama in Huntsville Laboratory for Structural Biology)
- Principle of the PCR
- PCR animation (The page takes a while to launch but it’s really worth waiting. BEST ANIMATION TO DELIVER THE AMPLIFICATION CONCEPT)
- Another good animation
Please suggest more links and I will put them one by one for you…
Tuesday 27 March, 2007 at 8:58 pm |
Thank you but please I want to say that the sites of lectures of Dr Abdelgawad and Dr hamadallah werenot opened
Wednesday 28 March, 2007 at 12:58 am |
Dear Basma,
thank you for your inupt.
I have tested Dr. Abdel Gawad’s lecture page and the page is opening fine (click here).
If I understood correctly, you could not open the page. Once the page is opened, you can download the files and uncompress them to see the lectures.
On the other hand, I will transfer your concerns to Dr. Hamdallah Zedan and I hope that any technical issue will be resolved.
Saturday 7 April, 2007 at 10:17 pm |
thanx alot Dr RKA
i suggest that u put a small expalanation of PCR by yourself.
it wil help us more,the animations and the sites r good ,but explanation will does help.
thank u again.
Monday 16 April, 2007 at 2:17 pm |
dear Dr RKA
is the PCR a method for just amplification of DNA ?
what about the plasmid cloning method used for production of hormones like insulin?
can the PCR method be used for the same purpose?
Monday 30 April, 2007 at 6:00 pm |
I searched about the relation between PCR& Plasmid cloning and found out that:PCR is often used to amplify the gene, which can then be inserted into a vector (a vector is a piece of DNA which ‘carries’ the gene into the GMO) such as a plasmid (a circular DNA molecule) . The DNA can then be transferred into an organism (the GMO) where the gene and its product can be studied more closely. Expressing a cloned gene (when a gene is expressed the gene product (usually protein or RNA) is produced by the GMO) can also be a way of mass-producing useful proteins, for example medicines or the enzymes.
GMO= genetically modified organism
Monday 14 May, 2007 at 10:31 pm |
dear Dr Ramy
please make a comment on my last post about plasmid cloning and PCR.